Thomas R, Prabhu PD, Mathivanan J, Rohini, Sivakumar D, Jayakumar PN, Devi BI, Satish S, Sastry KV, Gopel R

Mol Cell Biochem. 2005 Mar;271(1-2):113-21..

Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bangalore, India.

Tumor-specific alterations at the RB1 gene locus in 30 human vestibular schwannomas including 10 NF2 and 20 sporadic cases were analysed. Southern blot analysis of DNA from these samples revealed loss of heterozygosity (LOH) at the RB1 locus in 6 of 24 informative cases (25%) compared to normal blood DNAs from the same patients. Northern blot analysis showed normal size RB1 mRNA in all the tumor samples. However, there was a 2-5-fold increase in the level of expression of the RB1 gene in all the tumor samples compared to the WI38 cell line which was used as control. Western blot analysis of the RB1 protein, pRb showed a 2.5-5-fold increase in the level of total pRb as compared to normal WI38 cell line. Sixty five to seventy five percent of the total pRb were in phosphorylated form in most tumors. The LOH at the RB1 gene locus suggests genetic instability in these patients. Further, increased levels of RB1 mRNA, total pRb and the phosphorylated form of pRb suggests that RB1 gene in these tumors may have anti-apoptotic function. These results suggest that the RB1 gene has a major role in the development of human vestibular schwannomas.<

This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Acoustic Neuroma Foundation website. This abstract is representative of a larger article that is indexed on Medline.

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